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KMID : 1147720090020040306
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Journal of Acupuncture and Meridian Studies
2009 Volume.2 No. 4 p.306 ~ p.308
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Antioxidant Activity and Cytotoxicity of the Traditional Indonesian Medicine Tahongai (Kleinhovia hospita L.) Extract
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Arung Enos Tangke
Kusuma Irawan Wijaya
Purwatiningsih Sri
Roh Seong-Soo
Yang Chae-Ha
Jeon Soo-Hyeon
Kim Yong-Ung
Sukaton Edi
Susilo Joko
Astuti Yuli
Wicaksono Britanto Dani
Sandra Ferry
Shimizu Kuniyoshi
Kondo Ryuichiro
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Abstract
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We investigated the leaves of Kleinhovia hospita, a plant which has been traditionally used in Indonesia as phytotherapy to cure liver disease, to describe antioxidant materials from plant sources. K. hospita leaves were extracted with methanol and further partitioned into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and their cytotoxicity on HepG2 liver cancer cells was determined by a MTT assay. The K. hospita leaf methanol extract showed strong antioxidant activity (96%) compared with vitamin C (98%) by the DPPH method and the measured activity from the subsequent extracts of the methanol extract were 48.9% for n-hexane, 74.0% for diethyl ether, 84.3% for ethyl acetate, and 77.1% for the residue. The MTT assay showed the cytotoxicity of the methanol extract on HepG2 cells at 14%, 76%, and 80% at concentrations of 50μg/mL, 87.5μg/mL, and 125μg/mL, respectively. Leaf extracts of the medicinal plant K. hospita showed potent antioxidant activity and moderate cytotoxicity on HepG2 liver cancer cells.
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KEYWORD
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antioxidant activity, cytotoxicity, Kleinhovia hospita, traditional medicine
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